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Stable isotope-assisted metabolomics to detect metabolic flux changes in mammalian cell cultures
Institution:1. Modern Research Center for Traditional Chinese Medicine, the Institute for Biomedicine and Health, the Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, the Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province, Shanxi University, Taiyuan 030006, China;2. Department of Psychiatry, First Hospital/First Clinical Medical College of Shanxi Medical University, Taiyuan 030001, China
Abstract:
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  • Highlights? Stable isotope-assisted metabolomics is powerful to determine metabolic fluxes. ? Stable isotope-assisted metabolomics is more complex for mammalian cell cultures. ? Biotechnological processes can be improved by assessing metabolic flux changes. ? Metabolic flux analysis is an attractive tool for toxicity studies. ? There is a huge potential for future applications on mammalian systems.
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