Differences in TLR9-dependent inhibitory effects of H2O2-induced IL-8 secretion and NF-kappa B/I kappa B-alpha system activation by genomic DNA from five Lactobacillus species |
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Institution: | 1. Department of Neurology, Tenri Hospital, Tenri, Japan;2. Department of Clinical Pathology, Tenri Hospital, Tenri, Japan;1. Laboratoire de Bactériologie-Hygiène, département de Virologie, Bactériologie-Hygiène, Parasitologie-Mycologie, Assistance Publique-Hôpitaux de Paris, Centre Hospitalier Universitaire Henri Mondor, Créteil, France;2. Université Paris Est Créteil, Créteil, France;3. Unité Mixte de Recherche Dynamyc, Université Paris Est Créteil-École Nationale Vétérinaire d''Alfort, Maisons-Alfort, France;1. Service Universitaire des Maladies infectieuses et du Voyageur, Centre Hospitalier, Tourcoing, France;2. Centre régional de Pharmacovigilance, Centre Hospitalo-Universitaire, Lille, France |
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Abstract: | Lactic acid bacteria (LAB) show anti-inflammatory effects, and their genomic DNA was identified as one of the anti-inflammatory components. Despite the differences in anti-inflammatory effects between live LAB dependent not only on genus but also species, this effect has not been compared at the genomic DNA level. We compared the anti-inflammatory effects of the genomic DNA from five Lactobacillus species—Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus gasseri, Lactobacillus plantarum, and Lactobacillus reuteri—using Caco-2 cells. To evaluate anti-inflammatory effects, decreases in H2O2-induced IL-8 secretion and inhibition of H2O2-induced NF-κB/IκB-α system activation were examined. All LAB genomic DNAs dose-dependently decreased H2O2-induced IL-8 secretion and inhibited H2O2-induced NF-κB/IκB-α system activation. Comparison of these effects between Lactobacillus species showed that the anti-inflammatory effects of L. acidophilus genomic DNA are lower than those of the other species. Furthermore, suppression of Toll-like receptor 9 (TLR9), a specific receptor of bacterial DNA, expression by RNAi abolished the decrease of H2O2-induced IL-8 secretion and inhibition of H2O2-induced NF-κB/IκB-α system activation by LAB genomic DNA. Our results demonstrated that the anti-inflammatory effects of genomic DNA differ between Lactobacillus species and TLR9 is one of the major pathways responsible for the anti-inflammatory effect of LAB genomic DNA. |
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