Ultrastructure of putative germ granules in the penaeid shrimp Marsupenaeus japonicus |
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| Institution: | 1. Central Michigan University, Dept. of Biology, Brooks 217, Mount Pleasant, MI 48858, USA;2. CSIRO Food Futures National Research Flagship, CSIRO Livestock Industries, 306 Carmody Road, St Lucia, QLD 4067, Australia;3. CSIRO Food Futures National Research Flagship, CSIRO Marine and Atmospheric Research, 41 Boggo Road, Dutton Park, QLD 4102, Australia;1. Natural History Museum Vienna, 2nd Zoological Department, Burgring 7, 1010 Vienna, Austria;2. University of Vienna, Department of Integrative Zoology, Althanstrasse 14, 1090 Vienna, Austria;1. Allgemeine und Systematische Zoologie, Zoologisches Institut und Museum, Ernst-Moritz-Arndt-Universität, J.-S.-Bach-Str. 11/12, 17489 Greifswald, Germany;2. Department of Invertebrate Systematics and Ecology, Institute of Biology, Wroc?aw University of Environmental and Life Sciences, Ko?uchowska 5b, 51-631 Wroc?aw, Poland;1. Biology Department, Lund University, SE-22362 Lund, Sweden;2. Scripps Institution of Oceanography, University of California, La Jolla, CA 92093-0202, USA;1. Humboldt-Universität zu Berlin, Institut für Biologie/Vergleichende Zoologie, Philippstrasse 13, D-10115 Berlin, Germany;2. Raffles Museum of Biodiversity Research, Department of Biological Sciences, Faculty of Science, National University of Singapore, 6 Science Drive 2, Singapore 117546, Singapore;3. Landcare Research, 231 Morrin Rd, Tamaki Campus, University of Auckland, Private Bag 92170, Auckland, New Zealand;1. Programa de Pós-graduação em Biologia Celular e Estrutural, Departamento de Biologia Geral, Universidade Federal de Viçosa (DBG/UFV), Campus Universitário, Viçosa, Minas Gerais CEP 36570-000, Brazil;2. Department of Entomology, Kansas State University (KSU), Manhattan, KS 66506, USA |
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| Abstract: | Knowledge about the specification of the germ line in penaeid shrimp would allow development of techniques to control germ cell formation and/or fate to produce reproductively sterile shrimp for genetic copyright purposes. Recent studies have traced the localization of an RNA–enriched intracellular body (ICB) in the putative germ line of four penaeid shrimp species. It is hypothesized that the ICB may serve as a putative germ granule and marker of germ line fate. In this study semi-thin and ultra-thin sections of Marsupenaeus japonicus embryos were prepared, and the dimensions and ultrastructure of the ICB was examined at different stages of embryogenesis. The ICB was an aggregation of electron dense granules, small vesicles and multi-vesicular bodies (MVBs), similar to germ granules from other species. Lamellar membranes and mitochondria were localized at the periphery of the ICB. Using fluorescence microscopy, microtubules were also observed between the centrosome and the ICB. The localization of the ICB in the D lineage and putative germ cell line, the enrichment of RNA in the ICB, and the ultrastructural similarities to other germ granules characterized in this study support the hypothesis that the ICB contains germ granules. |
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