Biomolecular magic-angle spinning solid-state NMR: recent methods and applications
Institution:
1. Key Laboratory of Materials for High-Power Laser, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China;2. Institut für Physikalische Chemie, Westfälische Wilhelms-Universität Münster, Corrensstr. 30, D-48149 Münster, Germany;3. Instituto de Física de São Carlos, Universidade de São Paulo (USP), C.P. 369, CEP 13560-970, São Carlos, SP, Brazil;1. Beijing Nuclear Magnetic Resonance Center, Peking University, Beijing 100871, China;2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;3. Department of Physics, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada;4. Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1, Canada;1. School of Chemistry, Tel Aviv University, Ramat Aviv 69978, Tel Aviv, Israel;2. Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, United States;1. School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel;2. Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
Abstract:
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Highlights? Recent progress in biomolecular magic angle spinning solid state NMR is reviewed. ? MAS NMR is applied to study non-crystalline, non-soluble and high molecular-weight systems. ? New methods rely on sparse isotope enrichment, proton detection, fast spinning and signal enhancements. ? New structure calculations are based on 1H–1H restraints, paramagnetic effects and tensor analysis. ? Applications to protein aggregates, assemblies, fibrils, membrane proteins and enzymes are described.
