Rapid construction of genome map for large yellow croaker (Larimichthys crocea) by the whole-genome mapping in BioNano Genomics Irys system |
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Authors: | Shijun Xiao Jiongtang Li Fengshou Ma Lujing Fang Shuangbin Xu Wei Chen Zhi Yong Wang |
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Affiliation: | .Key Laboratory of Healthy Mariculture in the East China Sea, Ministry of Agriculture; Fisheries College, Jimei University, Yindou Road, Xiamen, P.R. China ;.Chinese Academy of Fishery Sciences, Yongding Road, Beijing, P.R. China ;.Genergy Inc., Shanghai, P.R. China |
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Abstract: | BackgroundLarge yellow croaker (Larimichthys crocea) is an important commercial fish in China and East-Asia. The annual product of the species from the aqua-farming industry is about 90 thousand tons. In spite of its economic importance, genetic studies of economic traits and genomic selections of the species are hindered by the lack of genomic resources. Specifically, a whole-genome physical map of large yellow croaker is still missing. The traditional BAC-based fingerprint method is extremely time- and labour-consuming. Here we report the first genome map construction using the high-throughput whole-genome mapping technique by nanochannel arrays in BioNano Genomics Irys system.ResultsFor an optimal marker density of ~10 per 100 kb, the nicking endonuclease Nt.BspQ1 was chosen for the genome map generation. 645,305 DNA molecules with a total length of ~112 Gb were labelled and detected, covering more than 160X of the large yellow croaker genome. Employing IrysView package and signature patterns in raw DNA molecules, a whole-genome map of large yellow croaker was assembled into 686 maps with a total length of 727 Mb, which was consistent with the estimated genome size. The N50 length of the whole-genome map, including 126 maps, was up to 1.7 Mb. The excellent hybrid alignment with large yellow croaker draft genome validated the consensus genome map assembly and highlighted a promising application of whole-genome mapping on draft genome sequence super-scaffolding. The genome map data of large yellow croaker are accessible on lycgenomics.jmu.edu.cn/pm.ConclusionUsing the state-of-the-art whole-genome mapping technique in Irys system, the first whole-genome map for large yellow croaker has been constructed and thus highly facilitates the ongoing genomic and evolutionary studies for the species. To our knowledge, this is the first public report on genome map construction by the whole-genome mapping for aquatic-organisms. Our study demonstrates a promising application of the whole-genome mapping on genome maps construction for other non-model organisms in a fast and reliable manner.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1871-z) contains supplementary material, which is available to authorized users. |
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Keywords: | Large yellow croaker Gene map Whole-genome mapping Genome assembly |
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