Association of Nitric Oxide Synthase and Matrix Metalloprotease Single Nucleotide Polymorphisms with Preeclampsia and Its Complications |
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| Authors: | Daniela P. Leonardo Dulcinéia M. Albuquerque Carolina Lanaro Letícia C. Baptista José G. Cecatti Fernanda G. Surita Mary A. Parpinelli Fernando F. Costa Carla F. Franco-Penteado Kleber Y. Fertrin Maria Laura Costa |
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| Affiliation: | 1. Hematology and Hemotherapy Center, University of Campinas—UNICAMP, Campinas, São Paulo, Brazil.; 2. Department of Obstetrics and Gynaecology, School of Medicine, University of Campinas—UNICAMP, Campinas, São Paulo, Brazil.; 3. Department of Clinical Pathology, School of Medicine, University of Campinas–UNICAMP, Campinas, São Paulo, Brazil.; University Francisco de Vitoria School of Medicine, SPAIN, |
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| Abstract: | BackgroundPreeclampsia is one of the leading causes of maternal and neonatal morbidity and mortality in the world, but its appearance is still unpredictable and its pathophysiology has not been entirely elucidated. Genetic studies have associated single nucleotide polymorphisms in genes encoding nitric oxide synthase and matrix metalloproteases with preeclampsia, but the results are largely inconclusive across different populations.ObjectivesTo investigate the association of single nucleotide polymorphisms (SNPs) in NOS3 (G894T, T-786C, and a variable number of tandem repetitions VNTR in intron 4), MMP2 (C-1306T), and MMP9 (C-1562T) genes with preeclampsia in patients from Southeastern Brazil.MethodsThis prospective case-control study enrolled 77 women with preeclampsia and 266 control pregnant women. Clinical data were collected to assess risk factors and the presence of severe complications, such as eclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome.ResultsWe found a significant association between the single nucleotide polymorphism NOS3 T-786C and preeclampsia, independently from age, height, weight, or the other SNPs studied, and no association was found with the other polymorphisms. Age and history of preeclampsia were also identified as risk factors. The presence of at least one polymorphic allele for NOS3 T-786C was also associated with the occurrence of eclampsia or HELLP syndrome among preeclamptic women.ConclusionsOur data support that the NOS3 T-786C SNP is associated with preeclampsia and the severity of its complications. |
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