Current developments in stepwise edman degradation of peptides and proteins |
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| Affiliation: | 1. Membrane Biophysics Group, Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17, 2100 Copenhagen Ø, Denmark;2. Division of Water Resources Engineering, Faculty of Engineering LTH, Lund University, John Ericssons Väg 1, V-Hus, Box 118, 221 00 Lund, Sweden;3. Grupo de Física de Coloides y Polímeros, Departamento de Física de Partículas, Universidad de Santiago de Compostela, Rúa Xosé María Suárez Núñez, s/n. Campus Vida, 15782 Santiago de Compostela, Spain;4. Sweden Water Research AB, Ideon Science Park, Scheelevägen 15, 22370 Lund, Sweden;1. University of Messina, Messina, Italy;2. University Campus Bio-Medico of Rome, Rome, Italy;3. Chromaleont SrL, Messina, Italy |
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| Abstract: | - 1.1. Since Edman's (1950, 1956) first publications about 30 years ago, the stepwise degradation of proteins and peptides is universally performed by protein chemists. We review the mechanism of the chemical reactions, and the different special problems encountered, during degradation and different manual methods of degradation.
- 2.2. We take one example of an alternative method using DABITC manually for the degradation of peptides in order to illustrate the evolution of manual degradation techniques (Chang, 1983).
- 3.3. Possibilities and limits of the liquid phase sequenator of Edman and Begg (1967), solid phase sequencer of Laursen (1975) and gas-liquid sequenator of Hewick et al. (1981) and those of Hunkapiller et al. (1983) are considered in detail.
- 4.4. We describe different procedures for identification of PTH-AA or DABTH-AA: thin layer chromatography, gas chromatography, high performance liquid chromatography, etc., in order to illustrate the evolution of the procedures of identification.
- 5.5. We conclude by taking two manual examples and two automatic procedures of degradation to underline the progress over the last decade.
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