Increased insulin responsiveness in isolated rat hepatocytes incubated with free fatty acid-poor albumin. |
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| Authors: | J F Caro J Hodges M K Sinha |
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| Affiliation: | Department of Medicine, East Carolina University School of Medicine, Greenville, North Carolina. |
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| Abstract: | Freshly isolated rat hepatocytes incubated in a medium containing bovine serum albumin partially purified by charcoal treatment at pH 3 are three times more responsive to insulin with regard to alpha-aminoisobutyric acid uptake than those incubated in medium containing regular bovine serum albumin. This finding should facilitate future work in isolated hepatocytes, a cell system that for the most part has been considered relatively unresponsive to insulin. |
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