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Phylogenetic analysis based evolutionary study of 16S rRNA in known Pseudomonas sp |
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| Authors: | Arindam Adhikari Suvodip Nandi Indrabrata Bhattacharya Mithu De Roy Tanusri Mandal Subrata Dutta |
| Affiliation: | 1.Department of Biotechnology, O.I.S.T. Vidyasagar University, Paschim Medinipur, India;2.AICRP on Vegetables crops, Directorate of research, BCKV, Kalyani, India;3.Department of Plant Pathology, BCKV, Mohanpur, Nadia, India;4.Soil Testing Laboratory, PORS, Berhampore, Murshidabad, India |
| Abstract: | Molecular evolution analysis of 16S rRNA sequences of native Pseudomonas strains and different fluorescent pseudomonads wereconducted on the basis of Molecular Evolutionary Genetics Analysis version 5.2 (MEGA5.2). Topological evaluations showcommon origin for native strains with other known strains with available sequences at GenBank database. Phylogenetic affiliationof different Pseudomonas sp based on 16S rRNA gene shows that molecular divergence contributes to the genetic diversity ofPseudomonas sp. Result indicate direct dynamic interactions with the rhizospheric pathogenic microbial community. The selectionpressure acting on 16S rRNA gene was related to the nucleotide diversity of Pseudomonas sp in soil rhizosphere community amongdifferent agricultural crops. Besides, nucleotide diversity among the whole population was very low and tajima test statistic value(D) was also slightly positive (Tajima׳s test statistics D value 0.351). This data indicated increasing trends of infection of soil-bornepathogens under gangetic-alluvial regions of West Bengal due to high degree of nucleotide diversity with decreased population ofplant growth promoting rhizobacteria like fluorescent Pseudomonads in soil. |
| Keywords: | Molecular diversity Positive selection 16S rRNA MEGA5.2 Pseudomonas sp |