An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: Nutritional factors affecting protease production |
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| Institution: | 1. Enzyme and Microbial Technology Research, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia;2. Enzyme and Microbial Technology Research, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia;1. Stem Cell Unit, Institute of Life Sciences, The Hebrew University of Jerusalem, Israel;2. Department of Genetics, Institute of Life Sciences, The Hebrew University of Jerusalem, Israel;3. Massachusetts General Hospital Cancer Center and Center for Regenerative Medicine, 185 Cambridge Street, Boston, MA 02114, USA;1. Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel;2. Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA |
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| Abstract: | An organic solvent-tolerant bacterium producing an organic solvent-stable protease was isolated from soil and identified as Pseudomonas aeruginosa strain K. Nutritional requirements for optimized protease production by this strain were investigated. Maximum protease activity was achieved with sorbitol as the sole carbon source, followed by starch and lactose at pH 7.0 and 37 °C. Dextrose, sucrose and glycerol greatly reduced the protease production. The best organic nitrogen source was casamino acid. Tryptone, soytone and yeast extract supported protease production while corn steep liquor and beef extract inhibited the protease activity. Significant protease production was observed with sodium nitrate as a sole nitrogen source however, ammonium nitrate completely inhibit it. More than 62% drop in production occurred in the presence of amino acids. Addition of metal ions such as K+, Mg2+ and Ca2+ maximized the enzyme production. |
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