Diauxic growth of Penicillium camembertii on glucose and arginine |
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| Affiliation: | 1. Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama-cho, Ikoma, Nara 630-0192, Japan;2. Technical Research Laboratories, Kyowa Hakko Bio Co. Ltd., 1-1 Kyowa-cho, Hofu, Yamaguchi 747-8522, Japan;3. MS Business Unit, Shimadzu Co., 1 Nishinokyo, Kuwabara-cho, Nakagyo-ku, Kyoto 604-8511, Japan;4. Department of Biochemistry, Keio University School of Medicine, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan;1. Departamento de Química-Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14040-901, SP, Brazil;2. Departamento de Bioquímica e Imunologia- Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, Ribeirão Preto, SP, Brazil;1. School of Chemical Engineering, University of Adelaide, SA 5005, Australia;2. Hardy Wines, Reynell Road, Reynella, SA 5161, Australia;1. Center for Preventive Doping Research/Institute of Biochemistry, German Sport University Cologne, Germany;2. Départment Santé, CER Groupe, Belgium;3. European Monitoring Center for Emerging Doping Agents (EuMoCEDA), Cologne/Bonn, Germany;1. Department of Biochemistry, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625021, Tamil Nadu, India;2. Department of Animal Science, Bharathidasan University, Trichy, Tamil Nadu 620024, India;3. Center for Molecular and Translational Medicine, Georgia State University, Petit Science Center, 100 Piedmont Ave., Atlanta, GA 30303, USA;4. Lead Discovery Group, Discovery Biologics, Chigene Biotech Private Limited, Sivagangai Tamil Nadu 630211, India;5. School of Chemistry, Madurai Kamaraj University, Madurai, Tamil Nadu 625021, India |
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| Abstract: | The growth of Penicillium camembertii during batch culture in a synthetic medium containing glucose and arginine was examined. The diauxic growth observed can be well characterized. Indeed, in a first phase, glucose and arginine were, respectively, assimilated as carbon and nitrogen sources, with an acidification of the medium (until 3.5), since arginine was taken up in exchange for protons. During this phase of growth, arginine, in addition to glucose, was also assimilated as an energy source, resulting in the release of the arginine carbon content as CO2. Then, in a second phase, characterized by reduced growth rates after glucose depletion, arginine was assimilated as a carbon and nitrogen source, as well as an energy source, resulting in ammonium release which raised the pH (final pH 6.3), despite the amino acid/H+ exchange, since amino acids contain excess nitrogen in relation to their carbon content for fungi. |
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