Attacin gene sequence variations in different ecoraces of tasar silkworm Antheraea mylitta

Attacin gene exists as paralogous conversion and is being used for identification of strain variations in insects based on thesequence variation. Hence, a study was undertaken to analyze the sequence variation of the attacin gene isoforms in the tasarsilkworm Anthereae mylitta that exists in the form of different ecoraces depending upon the environment, food plant and location.Comparison of the previously reported attacin sequences with the DNA sequences of attacin A and B genes revealed six aminoacid substitutions among the sequences of the ecoraces which however did not affect the functional domain of Attacin. Thegenerated dendrogram clearly indicated unique branches for each ecorace with two separate gene clusters for attacin A and B. TheSarihan ecorace formed a separate sub-group under both the gene clusters. The present study also revealed the presence ofAttacin_N Superfamily domain exclusively in Exon I separated from the Attacin_C Superfamily domain that was present in Exon IIand part of Exon III, a prominent character of attacin gene. The phylogenetic reconstruction analysis of attacin gene in A.mylittasupported the common evolutionary origin of attacin genes belonging to the Lepidoteran and Dipteran families that formed twoseparate clusters.