Production of medium chain glycerides from coconut and palm kernel fatty acid distillates by lipase-catalyzed reactions |
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| Institution: | 1. Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark;2. College of Life Science, Fujian Normal University, No. 1, Keji Road, Minhou, Fuzhou 350117, China;3. Engineering Research Center of Industrial Microbiology of Ministry of Education, Fujian Normal University, No. 1, Keji Road, Minhou, Fuzhou 350117, China;1. College of Life Science, Fujian Normal University, Fuzhou 350117, China;2. Engineering Research Center of Industrial Microbiology of Ministry of Education, Fujian Normal University, Fuzhou 350117, China;3. Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark;1. School of Food Science and Technology, Jiangnan University, Oil and Plant Protein Center, Wuxi, Jiangsu Province, PR China;2. Wuxi COFCO Engineering & Technology Co., Ltd, Wuxi, Jiangsu Province, PR China;1. School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China;2. Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China;3. Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, United States |
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| Abstract: | Medium chain glycerides (MCGs) containing C8:0 and C10:0 fatty acids is very much important for medicinal and nutritional applications. Coconut and palm kernel fatty acid distillates (FADs) can be utilized to produce MCGs by a combination of lipase-catalyzed hydrolysis and esterification reactions. The neutral glycerides present in coconut and palm kernel FADs are hydrolyzed by Candida rugosa lipase. The hydrolysates were then subjected to steam distillation under vacuum (at 120–140 °C) to get fractions rich in medium chain fatty acids (MCFAs). The fractions, from coconut and palm kernel FADs (75.2 and 76.2% MCFAs, respectively), were esterified with Rhizomucor miehei (Lipozyme RM IM) lipase to produce MCGs. Products from coconut FAD contained 64.7–67.5% diacylglycerol (DG), followed by 18.8–22.9% monoacylglycerol (MG) and 9.8–9.3% triacylglycerol (TG). Similarly, products from palm kernel FAD contained 63.5–66.7% DG, 19.1–23.6% MG and 9.5–10.1% TG. |
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