Simple and specific detection of Bordetella holmesii by using a loop-mediated isothermal amplification assay |
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| Authors: | Otsuka Nao Yoshino Shuji Kawano Kimiko Toyoizumi-Ajisaka Hiromi Shibayama Keigo Kamachi Kazunari |
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| Affiliation: | Department of Bacteriology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan. |
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| Abstract: | A loop-mediated isothermal amplification (LAMP) assay for simple detection of Bordetella holmesii was developed. This assay discriminates between B. holmesii and other Bordetella species and successfully detect B. holmesii DNA in nasopharyngeal swab samples from subjects with suspected pertussis. The LAMP assay results were in complete agreement with the results of previously published real-time PCR assay, indicating that the former is a powerful tool for the accurate diagnosis and surveillance of B. holmesii. |
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| Keywords: | Bordetella holmesii loop‐mediated isothermal amplification molecular diagnosis recA |
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