Isolation and characterization of hemorrhagic toxin from the venom of Trimeresurus elegans

1. Hemorrhagic toxin from the venom of Trimeresurus elegans was purified in a homogeneous form using gel filtration and ion exchange chromatographies. 2. Hemorrhagic toxin possessed hemorrhagic and TAME (tosyl-L-arginine methyl ester) hydrolytic activities. These activities were inhibited when hemorrhagic toxin was incubated with benzamidine or N-bromosuccinimide. 3. Its mol. wt was 28,500 and the isoelectric point was 7.4. 4. This toxin contains ca 1.5 mol Ca per mol of protein.