超声波降解紫球藻胞外多糖研究

通过测定溶液粘度,判断超声波降解紫球藻(Porphyridium cruentumi)胞外多糖的效果。运用均匀设计对超声波降解紫球藻胞外多糖的影响因素(处理振幅、时间、脉冲)进行优化,获得超声波处理的最佳条件:振幅39%、处理时间245s和脉冲9.5s。在最佳条件下,胞外多糖的粘度为2.98mm²/s,与预测值一致。采用DPS软件对实验结果进行二次多项式分析与拟合,并对模型和回归系数进行显著性检验,建立了以胞外多糖粘度为目标的回归方程式。

Ultrasonic Degradation of Extracellular Polysaccharide from Porphyridium cruentum

The effect of ultrasonic degradation of extracellular polysaccharide from Porphyridium cruentum was studied by determining the viscosity of solution. Uniform design method was used to optimize the parameter of ultrasonic, i.e. amplitude, irradiation time and pulse on the degradation of extracellular polysaccharide from Porphyridium cruentum. The optimal conditions for the viscosity were as follows:amplitude 39%, irradiation time 245s and pulse 9.5s. Under the optimal condition, the experimental value of the viscosity of the extracellular polysaccharide achieved to 2.98 mm²/s, compared to the calculated value, the error was 0%. Quadratic polynomial of DPS software was used to analyze and fit the result of the experiment, and the significance of model and regression coefficients was tested in order to establish a regression equation aimed at the viscosity of extracellular polysaccharide.