Comparison of the Efficiencies of Three Neural Induction Protocols in Human Adipose Stromal Cells |
| |
Authors: | Dong-Xiang Qian Hong-Tian Zhang Xu Ma Xiao-Dan Jiang Ru-Xiang Xu |
| |
Institution: | 1. Neurosurgery Institute of Guangdong, Key Laboratory on Brain Function Repair and Regeneration of Guangdong, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, 253# Gongye Road, 510282, Guangzhou, China 2. Department of Neurosurgery, The Third Affiliated Hospital, Guangzhou Medical College, 510150, Guangzhou, China 3. Department of Neurosurgery, The General Hospital of Beijing PLA, 100700, Beijing, China 4. Department of Neurosurgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, 510120, Guangzhou, China
|
| |
Abstract: | The aim of this study was to compare the neural differentiation potential and the expression of neurotrophic factors (NTFs) in differentiated adipose-derived stem cells (ADSCs) using three established induction protocols, serum free (Protocol 1), chemical reagents (Protocol 2), and spontaneous (Protocol 3) protocols. Protocol 1 produced the highest percentage of mature neural-like cells (MAP2ab+). Protocol 2 showed the highest percentage of immature neural-like cells (β-tubulin III+), but the neural-like state was transient and reversible. Protocol 3 caused ADSCs to differentiate spontaneously into immature neural-like cells, but not into mature neural cell types. The neural-like cells produced by Protocol 1 lived the longest in culture with little cell death, but Protocol 2 and 3 led to the significant cell death. Therefore, Protocol 1 is the most efficient among these protocols. Additionally, soon after differentiation, the mRNA levels of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in dADSCs were sharply decreased by Protocol 1 and 2 (acute induction protocol), but not by Protocol 3 (chronic induction protocol). The results indicate that NTFs played an important role in neural differentiation via acute responses to NGF and BDNF, but not chronically during the transdifferentiation process. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|