Influence of extremely-low-frequency magnetic field on antioxidative melatonin properties in AT478 murine squamous cell carcinoma culture |
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| Authors: | K ?wirska-Korczala M Adamczyk-Sowa R Polaniak P Sowa E Birkner Z Drzazga T Brzozowski S J Konturek |
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| Institution: | (1) Department of Physiology, Silesian University School of Medicine, Zabrze, Poland;(2) Department of Biochemistry, Silesian University School of Medicine, Zabrze, Poland;(3) A. Chelkowski Institute of Physics, Department of Medical Physics, University of Silesia, Katowice, Poland;(4) Department of Physiology, Jagiellonian University School of Medicine, Cracow, Poland |
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| Abstract: | Effects of melatonin, extremely-low-frequency magnetic field (ELF-MF), and their combination on AT478 murine squamous cell
carcinoma line were studied. Manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (Cu/ZnSOD), and glutathione
peroxidase (GSH-Px) were used as markers of cells antioxidative status, and malondialdehyde (MDA) level was used as a marker
of lipid peroxidation. After melatonin treatment, antioxidative enzyme activities were increased and MDA level was decreased.
Application of ELF-MF on treated cells caused an increase of both superoxide dismutases activity and MDA level, but influence
of ELF-MF on GSH-Px activity was negligible. All enzyme activity in culture medium containing melatonin (10−3, 10−4, 10−5
M) after exposure to ELF-MF were significantly diminished compared to cells treated only with melatonin. Also MDA levels after
combined treatment with melatonin and ELF-MF were significantly decreased. Observed changes were statistically significant
(p<0.05). These results strongly suggest that ELF-MF attenuates antioxidative actions of melatonin on cellular level. |
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| Keywords: | MnSOD Cu/ZnSOD GSH-Px MDA ELF-MF melatonin carcinoma cell culture |
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