Protein separation using metal ion-bound particles in aqueous two-phase system |
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Authors: | Yun Joong Kwon Rajni Hatti-Kaul |
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Institution: | (1) Department of Food and Biotechnology, Kyonggi University, Suwon, 442–760, Korea;(2) Department of Biotechnology, Center for Chemistry and Chemical Engineering, und University, 22100 Lund, Sweden |
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Abstract: | Metal ion affinity partitioning of protein in aqueous two-phase systems was studied using Sepharose as ligand carrier as an integrated adsorption partitioning. Cu(II)-bound Sepharose was mixed with protein solution and an aqueous two-phase system. The affinity sorbent was distributed quantitatively to the upper side or the interface. The binding studies of lysozyme to copper-bound gel in PEG/dextran two-phase systems demonstrate the feasibility of this bioseparation process. PEG/dextran system did not affect binding and elution of lysozyme to and from the Cu(II)-Sepharose particles. |
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Keywords: | protein separation aqueous two-phase system affinity partitioning lysozyme |
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