Structure of a New Pyrimidine from Bacillus subtilis Phage SP-15 Nucleic Acid |
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Authors: | C. BRANDON P. M. GALLOP J. MARMUR H. HAYASHI K. NAKANISHI |
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Affiliation: | 1.Department of Biochemistry,Albert Einstein College of Medicine,Bronx;2.Department of Chemistry,Colombia University New York |
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Abstract: | THE unusual modified base1 of the nucleic acid of the large, generalized transducing phage of Bacillus subtilis SP-15 was predicted from the low denaturation temperature of the native nucleic acid. The base was detected as a fifth ultraviolet-absorbing component once the nucleic acid had been hydrolysed with formic acid; it partially replaces the thymine and represents 12 mol per cent of all the bases1. It is responsible for the low Tm of the nucleic acid, since once all other unusual components have been removed, the remaining material melts at the same temperature as the native nucleic acid. We report here the isolation and characterization of this new pyrimidine. |
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