Biotechnological production of unnatural L-amino acids from D,L-5-monosubstituted hydantions. I. Derivatives of L-phenylalanine |
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Authors: | Christoph Syldatk Vera Lehmensiek Gaby Ulrichs Ulrich Bilitewski Karsten Krohn Hartmut Höke Fritz Wagner |
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Affiliation: | (1) Institute of Biochemistry and Biotechnology A, Technical University of Braunschweig, D-3300 Braunschweig, FRG;(2) Institute of Organic Chemistry, Technical University of Braunschweig, D-3300 Braunschweig, FRG;(3) Rütgers-BioTech, D-6800 Mannheim, FRG |
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Abstract: | Summary Resting cells of a mutant ofArthrobacter sp. (DSM 3747) were used for the bioconversion of D,L-5-benzylhydantoin and related compounds to the corresponding L-amino acids. After optimization of the reaction conditions in shake flask experiments, bioconversions were performed in a preparative scale in a 2-l-bioreactor under nitrogen atmosphere. Specific productivities of 0.4 (p-NO2-L-phenylalanine) up to 3.9 mM amino acid x g cell dry mass–1 x h–1 (p-Cl-L-phenylalanine) were obtained. D,L-5-p-COOH-Benzylhydantoin, D,L-5-phenylhydantoin and D,L-5-p-OH-phenylhydantoin were not accepted as substrates. |
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