Carbon Disulfide-Induced Changes in Cytoskeleton Protein Content of Rat Cerebral Cortex |
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Authors: | Fuyong Song Sufang Yu Xiulan Zhao Cuili Zhang Keqin Xie |
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Institution: | (1) Institute of Toxicology, Shandong University, 44 West Wenhua Road, Jinan, Shandong, 250012, P. R. China |
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Abstract: | To investigate the mechanism of carbon disulfide-induced neuropathy, male wistar rats were administrated by gavage at dosage
of 300 or 500 mg/kg carbon disulfide, five times per week for 12 weeks. By the end of the exposure, the animals produced a
slight or moderate level of neurological deficits, respectively. Cerebrums of carbon disulfide-intoxicated rats and their
age-matched controls were Triton-extracted and centrifuged at a high speed (100,000 × g) to yield a pellet fraction of NF polymer and a corresponding supernatant fraction, which presumably contained mobile monomer.
Then, the contents of six cytoskeletal protein (NF-L, NF-M, NF-H, α-tubulin, β-tubulin, and β-actin) in both fractions were
determined by immunoblotting. Results showed that the contents of the three neurofilament subunits in the pellet and the supernatant
fraction decreased significantly regardless of dose levels (P < 0.01). As for microtubule proteins, in the pellet fraction of cerebrum, the levels of α-tubulin and β-tubulin demonstrated
some inconsistent changes. However, in the supernatant fractions, the content of α-tubulin and β-tubulin increased significantly
in both two dose groups (P < 0.01). In comparison to neurofilament and tubulin proteins, the content of β-actin changed less markedly, only the supernatant
fraction of the high dose group displayed significant increase (P < 0.01), but the others remained unaffected. These findings suggested that the changes of cytoskeleton protein contents in
rat cerebrum were associated with the intoxication of carbon disulfide, which might be involved in the development of carbon
disulfide neurotoxicity. |
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Keywords: | Carbon disulfide cytoskeletal proteins distal axonpathy neurofilaments |
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