| 氨基葡萄糖硫酸盐诱导凋亡中Cathepsin D与Bcl-xL的细胞内共定位研究 |
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| 引用本文： | 臧丹丹,闫庆国,王哲,师建国,王文勇,王映梅,黄高昇. 氨基葡萄糖硫酸盐诱导凋亡中Cathepsin D与Bcl-xL的细胞内共定位研究[J]. 现代生物医学进展, 2010, 10(1) |
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| 作者姓名： | 臧丹丹 闫庆国 王哲 师建国 王文勇 王映梅 黄高昇 |
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| 作者单位： | 第四军医大学病理与病理生理学教研室暨西京医院病理科,陕西,西安,710032 |
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| 基金项目： | 国家自然科学基金资助项目(30770903) |
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| 摘 要： | 目的:探讨氨基葡萄糖硫酸盐(Glucosamine Sulfate,GS)诱导K562细胞凋亡中溶酶体Cathepsin D释放后与Bcl-xL的细胞内共定位关系。方法:采用5.0mmol.L-1 GS诱导K562细胞凋亡,通过HE染色和流式细胞仪检测细胞凋亡,应用免疫荧光染色结合激光共聚焦显微镜观察5.0mmol.L-1 GS诱导凋亡前后Cathepsin D与Bcl-xL的细胞内共定位关系。结果:GS诱导K562细胞72小时后出现细胞凋亡的形态改变,流式细胞仪检测表明细胞出现凋亡,诱导凋亡后的Cathepsin D与Bcl-xL的细胞内共定位信号增强。结论:GS诱导K562细胞凋亡后Cathepsin D与Bcl-xL存在细胞内共定位关系,具备一定相互作用的空间基础。
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| 关 键 词： | 氨基葡萄糖硫酸盐 凋亡 Cathepsin D Bcl-xl 共定位 |
The colocalization study of Cathepsin D and Bcl-xl in apoptosis of chronic myelogenous leukemia K562 cell induced by glucosamine sulfate |
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| ZANG Dan-dan,YAN Qing-guo,WANG Zhe,SHI Jian-Guo,WANG Wen-Yong,WANG Ying-mei,HUANG Gao-Sheng. The colocalization study of Cathepsin D and Bcl-xl in apoptosis of chronic myelogenous leukemia K562 cell induced by glucosamine sulfate[J]. Progress in Modern Biomedicine, 2010, 10(1) |
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| Authors: | ZANG Dan-dan YAN Qing-guo WANG Zhe SHI Jian-Guo WANG Wen-Yong WANG Ying-mei HUANG Gao-Sheng |
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| Affiliation: | ZANG Dan-dan,YAN Qing-guo,WANG Zhe,SHI Jian-Guo,WANG Wen-Yong,WANG Ying-mei,HUANG Gao-Sheng(Department of Pathology , pathophysiology,The Fourth Military Medical University,Xi'an 710032,China) |
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| Abstract: | Objective:To study the colocalization of Cathepsin D and Bcl-xl in apoptosis of chronic myelogenous leukemia K562 cell induced by glucosamine sulfate.Methods:The apoptosis of K562 cells induced by 5.0 mm.L-1 glucosamine sulfate were detected by heamatoxylin eosin staining and flow cytometric analysis.The colocalization of Cathepsin D and Bcl-xl in K562 cells induced by 5.0 mm.L-1 glucosamine sulfate was observed by by laser confocal scanning microscope after fimmunofluorescence staining.Results:After induce... |
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| Keywords: | Glucosamine sulfate Apoptosis Cathepsin D Bcl-xl Colocalization |
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