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Complete nucleotide sequence of the messenger RNA coding for chicken muscle glyceraldehyde-3-phosphate dehydrogenase
Authors:F Panabières  M Piechaczyk  B Rainer  C Dani  P Fort  S Riaad  L Marty  J L Imbach  P Jeanteur  J M Blanchard
Affiliation:1. Laboratoire de Biologie Moléculaire, Université des Sciences et Techniques du Languedoc, Place E. Bataillon, 34060 Montpellier, France;2. Laboratoire de Biochimie, Centre Paul Lamarque, BP 5054, 34033, Montpellier, France;1. Laboratoire de chimie Bio-Organique, Université des Sciences et Techniques du Languedoc, Place E. Bataillon, 34060 Montpellier, France
Abstract:The complete nucleotide sequence for chicken glyceraldehyde-3-phosphate dehydrogenase mRNA has been determined, thereby extending the longest such sequence previously reported (Dugaiczyk et al. Biochemistry, 1983, 22, 1605-1613) by 27 nucleotides. The complete mRNA with the exclusion of poly(A) is 1284 nucleotides long and contains 56 nucleotides of 5' non coding sequence and 229 nucleotides of 3' non coding region. Knowledge of the complete sequence allows us to propose secondary structures models which may be of biological significance.
Keywords:Hepes  4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid  EGTA  ethylene glycol bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid  SDS-PAGE  sodium dodecyl sulfate polyacrylamide gel electrophoresis
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