Affiliation: | (1) Marine Biotechnology Institute, Heita 3-75-1, Kamaishi, Iwate 026-0001, Japan;(2) Fisheries Experiment Station, Yaeyama Branch of Okinawa Prefectural Government Laboratory, Kabira 828-2, Ishigakishi, Okinawa 907-0453, Japan;(3) Japan Agency for Marine Earth Science and Technology, Marine Ecosystem Research Department, Natsushima 2-15, Yokosuka Kauagawa 237-0061, Japan;(4) Yokosuka City Museum, Yokosuka Kanagawa 238-0016, Japan |
Abstract: | Recent molecular biological studies have revealed that some photosymbiotic invertebrates dwelling in coral reefs host several genetically different dinoflagellates, Symbiodinium species, as symbionts. However, little is known about the difference in physiologic characteristics among these symbionts living in a single host, because some Symbiodinium strains are difficult to culture in vitro. To isolate some of these Symbiodinium strains, we have developed an agar culture medium plate containing antibiotics and a giant clam tissue homogenate. Using-this medium we isolated two new Symbiodinium strains from two molluscan hosts, Tridacna crocea and Pteraeolidia ianthina, each of which hosted two different Symbiodinium strains belonging to Symbiodinium C and D, respectively. The tissue homogenate was essential for the growth of Symbiodinium D. Although it was not essential for the growth of Symbiodinium C, it did stimulate the initial growth. For the isolation of some Symbiodinium strains, isolation medium containing host homogenate is effective. |