| RAP-PCR技术及其应用 |
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| 引用本文： | 李智,李强,孙春晓,于常海,LI Zhi,LI Qiang,SUN Chun-xiao,Albert C.H.Yu,. RAP-PCR技术及其应用[J]. 遗传, 2001, 23(4): 381-690 |
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| 作者姓名： | 李智 李强 孙春晓 于常海 LI Zhi LI Qiang SUN Chun-xiao Albert C.H.Yu |
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| 作者单位： | 1.中国科学院上海生命科学研究中心上海 200031;2.香港科技大学生物系,香港1.Laboratory of Neuronal Injury and Regeneration,Shanghai Research Center of Life Sciences,Chinese Academy of Sciences,Shanghai 200031,China;2.Department of Biology,Hong Kong University of Sciences and Technology,Hong Kong,China |
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| 摘 要： | RAP-PCR是以PCR为基础构建RNA指纹图谱,研究基因差异表达的有效方法.所显示的种系特异性差异可用于对基因的遗传作图;所揭示的组织特异性差异可用于研究特异基因的表达.该法可检测各种情形下RNA群体间的差异.本文简介其基本原理及在基因差异表达研究领域的最新应用。Abstract:RAP-PCR is a useful method based on PCR to display fingerprint of RNA populations and to study differential gene expression.The strain-specific differences revealed should be useful for genetic mapping of genes.The tissue-spe-cific differences revealed might be useful for studying differential gene expression.In this review,we introduce the theory of RAP-PCR and its recent applications.
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| 关 键 词： | RAP-PCR differential gene expression 基因克隆 Key words 基因差异表达 RAP-PCR |
RAP-PCR,from Theory to Applications |
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| LI Zhi,LI Qiang,SUN Chun-xiao,Albert CHYu. RAP-PCR,from Theory to Applications[J]. Hereditas, 2001, 23(4): 381-690 |
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| Authors: | LI Zhi LI Qiang SUN Chun-xiao Albert CHYu |
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