Quantitative detection of the oil-degrading bacterium Acinetobacter sp. strain MUB1 by hybridization probe based real-time PCR |
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Authors: | Phrommanich Seksan Suanjit Sudarat Upatham Suchart Grams Suksiri Vichasri Kruatrachue Maleeya Pokethitiyook Prayad Korge Günter Hofmann Annemarie |
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Affiliation: | Biological Science Graduate Program, Faculty of Science, Burapha University, Bangsaen, Chonburi, Thailand. seksan55@yahoo.com |
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Abstract: | Quantitative detection of the oil-degrading bacterium Acinetobacter sp. strain MUB1 was performed using the SoilMaster™ DNA Extraction Kit (Epicentre, Madison, Wisconsin) and hybridization probe based real-time PCR. The detection target was the alkane hydroxylase gene (alkM). Standard curve construction showed a linear relation between log values of cell concentrations and real-time PCR threshold cycles over five orders of magnitude between 5.4±3.0×106 and 5.4±3.0×102 CFU ml−1 cell suspension. The detection limit was about 540 CFU ml−1, which was ten times more sensitive than conventional PCR. The quantification of Acinetobacter sp. strain MUB1 cells in soil samples resulted in 46.67%, 82.41%, and 87.59% DNA recovery with a detection limit of 5.4±3.0×104 CFU g−1 dry soil. In this study, a method was developed for the specific, sensitive, and rapid quantification of the Acinetobacter sp. strain MUB1 in soil samples. |
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Keywords: | Acinetobacter sp. strain MUB1 Alkane hydroxylase gene (alkM) Hybridization probe based real-time PCR SoilMaster™ DNA Extraction Kit Quantitative detection |
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