Relative spraint density and genetic structure of otter (Lutra lutra) along the Drava River in Hungary |
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Authors: | J. Lanszki, A. Hidas, K. Szentes, T. R vay, I. Lehoczky,S. Weiss |
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Affiliation: | aEcological Research Group, University of Kaposvár, P.O. Box 16, 7401 Kaposvár, Hungary;bGenetic Laboratory, Research Institute for Animal Breeding and Nutrition, Isaszegi Str. 200, 2100 Gödöllö, Hungary;cGenetic Laboratory, Research Institute for Fisheries, Aquaculture and Irrigation, P.O. Box 47, 5541 Szarvas, Hungary;dInstitute of Zoology, Karl-Franzens University Graz, Universitätsplatz 2, 8010 Graz, Austria |
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Abstract: | In this study, we used genetic-based approaches to estimate population size and structure of Eurasian otter along the Drava River in Hungary, and compared these results to traditional survey-based methods. The relative spraint density of otter was estimated based on the number of fresh (Df) and total number (Dt) of spraints collected on standard routes over a 2-year period. Nine microsatellite loci were screened, generating 17 individual otter genotypes composed of 45 different alleles. The expected heterozygosity ranged from 0.53 to 0.89 and observed heterozygosity from 0.25 to 0.92. The mean density (Dg) estimated over six different sites was 0.17 individuals per km of shoreline. A close correlation was found between the number of genotypes and spraint counts along a standard route (fresh spraints: rP=0.85, P<0.01; total spraints rP=0.76, P<0.05). All genotypes found within the 50 km-long study area were closely related (Dm ranged between 0.08 and 0.21). |
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Keywords: | Lutra lutra Microsatellite loci Genotype Heterozygosity Density |
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