|
|||||
|
|
Cloning and characterization of a novel GH44 family endoglucanase from mangrove soil metagenomic library |
|
| Authors: | Zhimao Mai Hongfei Su Jian Yang Sijun Huang Si Zhang |
| Affiliation: | 1. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China 2. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China 3. South China Sea Institute of Ocean, Chinese Academy of Sciences, 164 Xingang Road, Guangzhou, 530104, People’s Republic of China |
| Abstract: | A novel endoglucanase gene, mgcel44, was isolated from a mangrove soil metagenomic library by functional-based screening. It encodes a 648-aa peptide with a catalytic domain of glycosyl hydrolase family 44. The deduced amino acid sequence of mgcel44 shares less than 50 % identity with endoglucanases in GenBank database. mgcel44 was cloned and overexpressed in Escherichia coli. The recombinant enzyme, MgCel44, has a molecular mass of 70.8 kDa as determined by SDS-PAGE. Its optimal pH and temperature for activity were 6 and 45 °C, respectively. It was highly active at 25–45 °C and pH 5–8. Its activity was enhanced in 0.5 M NaCl by >1.6-fold and stable up to 1.5 M NaCl. MgCel44 was resistant to several organic solvents and had high activity at 15 % (v/v) solvent after incubating for 24 h at 25 °C. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |