An assay for the bacterial sweet spot |
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| Authors: | Dr. Danielle Tullman-Ercek |
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| Affiliation: | Department of Chemical and Biomolecular Engineering, University of California Berkeley, Berkeley, CA, USA |
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| Abstract: | Glycosylation is critical for the proper folding and function of many eukaryotic proteins. A lack of native asparagine-linked glycosylation machinery has hampered production of eukaryotic proteins in Escherichia coli. This commentary by Danielle Tullman-Ercek discusses the work of Matt Delisa's group on an assay using beta-lactamase activity as a reporter for successful glycoprotein folding in the periplasm of E. coli. See accompanying article by Mansell et al. DOI: 10.1002/biot.201300026 |
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