Cloning of the genes encoding urease from Proteus vulgaris and sequencing of the structural genes |
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Authors: | Gerhard Mö rsdorf,Heinrich Kaltwasser |
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Affiliation: | Fachrichtung Mikrobiologie der Universit?t des Saarlandes, F.R.G. |
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Abstract: | A fragment of chromosomal DNA from proteus vulgaris encoding urease was cloned and expressed in Escherichia coli. A 3 kbp region was sequenced and revealed three open reading frames with homology to jack bean (Canavalia ensiformis) urease. The smallest protein (11 kDa) was homologous to the N-terminus of the plant enzyme and the largest polypeptide (61 kDa) corresponded to the C-terminus. The large protein contained conserved regions and a cysteine residue which is known to be catalytically important in the plant enzyme. A protein of 12 kDa showed homology to residues 132 to 237 of jack bean urease. |
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Keywords: | Urease Cloning Nucleotide sequence Proteus vulgaris |
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