Genetic interactions in the control of mitochondrial functions in Paramecium |
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Authors: | Françoise Ruiz and Janine Beisson |
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Institution: | (1) Centre de Génétique Moléculaire du C.N.R.S., 91190 Gif-sur-Yvette, France |
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Abstract: | Summary The genetic and physiological properties of two nuclear mutants of Parameccium tetraurelia affecting mitochondrial properties, and first screened as resistant to tetrazolium (TTC) are described. The mutant TTC
64-1
R
is strongly deficient in cytochrome c and the mutant TTC
66p
R
is partially deficient in cytochrome aa3; both mutants display cyanide insensitive respiration in exponential growth phase. In the double mutant TTC
64-1
R
-TTC
66p
R
/TTC
64-1
R
-TTC
66p
R
the deficiency in cytochrome aa3 due to the TTC
64-1
R
mutation is suppressed. The mutation TTC
64-1
R
does not suppress cytochrome aa3 deficiencies due to mitochondrial mutations, but does interact with another nuclear mutation, cl
1, (compatible only with mitochondria deficient in cytochrome oxidase) in such a way that the double mutant TTC
64-1
R
-cl
1/TTC
64-1
R
-cl
1 displays a normal amount of cytochrome aa3. The possible mechanisms and physiological significance of these suppressive effects are discussed.Abbreviations TTCR/TTCS
resistant/sensitive to tetrazolium
- KCNR/KCNS
cyanide insensitive/sensitive respiration
- aa
3
-
/aa
3
+
deficient/normal amount of cytochrome aa3
- c-/c+
deficient/normal amount of cytochrome c |
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Keywords: | |
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