HNCA+, HNCO+, and HNCACB+ experiments: improved performance by simultaneous detection of orthogonal coherence transfer pathways |
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Authors: | Sergio Gil-Caballero Adrien Favier Bernhard Brutscher |
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Institution: | 1. Institut de Biologie Structurale, Université Grenoble 1, 71 Avenue des Martyrs, 38044, Grenoble Cedex 9, France 2. Commissariat à l’Energie Atomique et aux Energies Alternatives (CEA), Grenoble, France 3. Centre National de Recherche Scientifique (CNRS), Grenoble, France
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Abstract: | Three experiments, BEST–TROSY HNCA+, HNCO+ and HNCACB+ are presented for sequential backbone resonance assignment of 13C, 15N labelled proteins. The novelty of these experiments with respect to conventional pulse sequences is the detection of additional orthogonal coherence transfer pathways that results in enhanced sensitivity for sequential correlations without significantly compromising the intensity of intra-residue correlation peaks. In addition, a 2-step phase cycle separates peaks originating from the orthogonal coherence transfer pathways in 2 sub-spectra, thus providing similar information as obtained from performing a pair of sequential and intra-residue correlation experiments. |
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