A colorimetric method for the quantitative determination of thiazolidine-4-carboxylic acids |
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| Authors: | G G Guidotti A F Borghetti L Loreti |
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| Affiliation: | 1. Brain and Mind Research Institute, Department of Neurology, Weill Cornell Medical College, New York, NY 10021, USA;2. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, Russia;3. Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA;1. Laboratorio de Farmacologia, Departamento de Ciencias de la Salud, DCBS, Universidad Autonoma Metropolitana-Iztapalapa (UAM-I), Avenida San Rafael Atlixco 186, A.P. 55-535, C.P. 09340, Mexico City, Mexico;2. Unidad de Investigacion Medica en Bioquimica (UIM), Hospital de Especialidades, Centro Medico Nacional Siglo XXI. IMSS, Av. Cuauhtemoc 330, Col. Doctores, Del. Cuauhtemoc, Mexico City, Mexico;3. Departamento de Medicina Genomica y Toxicologia Ambiental, IIB, UNAM, SZ, CDMX, Mexico;4. Unidad de Bioquimica Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran, Mexico City Mexico;5. Laboratorio Divisional de Microscopia Confocal, DCBS, Universidad Autonoma Metropolitana-Iztapalapa (UAM-I), Avenida San Rafael Atlixco 186, A.P. 55-535, C.P. 09340, Mexico City, Mexico;1. First Department of Internal Medicine, San Matteo Hospital Foundation, University of Pavia, Pavia, Italy;2. Department of Geriatric Care, OrthoGeriatrics and Rehabilitation, Frailty Area, Galliera Hospital, Genova, Italy |
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| Abstract: | A simple colorimetric method for the quantitative estimation of thiazolidine-4-carboxylic acids using 3-methyl-2-benzothiazolone hydrazone, iodine, and ferric chloride has been described. The sensitivity of the method is such that microgram quantities of thiazolidinecarboxylic acids can be measured rapidly and accurately. The procedure is suitable for the assay of TCA or PCA supernatants from tissue homogenates, incubation media, and blood. |
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