温州蜜柑萎缩病毒和柑桔衰退病毒在苗木各部位分布的全年分析

１９９１年９月至１９９２年８月逐月采样，采用ＤＡＳ－ＥＬＩＳＡ法对温州蜜柑萎缩病毒（ＳＤＶ）在温州蜜柑苗木各部位及对柑桔衰退病毒（ＣＴＶ）在甜橙苗木各部位的分布进行了全年分析。结果表明ＳＤＶ在老叶、老枝皮中全年均检测不到；在根中仅在７、８月份可检出有少显病毒存在；在嫩叶中３、４、５、６、９、１０月均可检出且浓度较高，而７、８月在嫩叶中检测不出，在嫩枝皮中４、５、６、７、１０月均可检出而７月份浓度有较大幅度降低。这说明冷凉温度下的嫩组织适立于ＳＤＶ的繁殖，最佳检测时期为春梢和秋梢的嫩梢期，最佳检测部位为幼嫩的叶和嫩枝皮。对ＣＴＶ在甜橙苗木各部位分布检测结果表明各部位全年均可检测出带毒且含量均较高，总的变幅不大，冬季和夏季含量有所下降，在老叶中变幅稍大些，这说明甜橙苗木各个部位均适宜于采样且全年均可进行分析。

Annual Change of ELISA Values of Satsuma Dwarf Virus in Different Parts of Satsuma Budling and of Citrus Tristeza Virus in Different Parts of Sweet Orange Seedling

rom September 1991 to August 1992,leaves,stem barks and roots collected in each monthfrom four-year-old satsuma budling infected with satsuma dwarf virus(SDV) and from four-year-oldsweet orange seedling infected with citrus tristza virus(CTV)were used for detecting SDV and CTVby using DAS-ELISA.The results show that SDV could not be detected in old leaves and stem barksall over the year.It could be stably detected in young leaves in March,April,May,June,Septemberand October but not in July and August,and in young steni barks it could be detected in April,May,June,July and Octuber,the concentration of SDV in young stem bark decreased sharply in July,Lowconcentratiun of SDV in roots could be detected in July and August as the new roots grow,This indicates that SDV propagated in young tissues under the terms of cool temperature.CTV could be stablydetedted not only in stem barks but also in leaves and roots all over the year,but the ELISA values(A410nm)decreased in summer and winter season,especially in old leaves, and in the other parts,thedecrease of ELISA values is sligtit.This report revealed that SDV could be detected only in youngshoots of satsuma growing under cool temperature but CTV could be detected in any part of sweet orange seedling all over the year.