A translocation activating the cryptic nitrogen regulation gene areB inactivates a previously unidentified gene involved in glycerol utilisation in Aspergillus nidulans |
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Authors: | Herbert N Arst Dirk H A Hondmann and Jaap Visser |
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Institution: | (1) Department of Bacteriology, Royal Postgraduate Medical School, Ducane Road, W12 ONN London, England;(2) Department of Genetics, Agricultural University, Dreijenlaan 2, NL-6703 HA Wageningen, The Netherlands |
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Abstract: | Summary The chromosome VIII translocation breakpoint of the areB-404 translocation, selected for its ability to activate the cryptic nitrogen metabolism regulatory gene areB, and the mutation glcD-100 both lead to loss of mitochondrial FAD-dependent sn-glycerol-3-phosphate dehydrogenase in Aspergillus nidulans. These two lesions therefore define glcD, a second gene (in addition to glcB) where mutation can result in loss of this enzyme. The glcD gene has been localised to a centromere-proximal region of the right arm of chromosome VIII. Although all six known areB-activating mutations involve chromosomal rearrangements and presumably therefore gene fusions, areB-404 is the first such rearrangement where the gene involved in an areB fusion has been identified. |
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Keywords: | Aspergillus nidulans Gene regulation Glycerol metabolism Nitrogen regulation Translocation |
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