Identification and Molecular Characterization of a New Geminivirus Betasatellite Infecting Malvastrum coromandelianum in China |
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Authors: | Yafei Tang Zifu He Zhenguo Du Xiaoman She Guobing Lan |
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Institution: | 1. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China;2. Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou, China |
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Abstract: | The complete nucleotide sequence of a satellite molecule associated with Malvastrum leaf curl Guangdong virus (MLCuGdV) infecting M. coromandelianum plants exhibiting leaf curl symptoms in a suburb of Guangzhou, Guangdong Province of China, is described and analysed. The molecule has typical features of betasatellites, containing a single ORF in the complementary‐sense strand, an A‐rich region, the satellite‐conserved region and a stem–loop structure. Compared with the geminivirus betasatellites in GenBank database, this molecule shows the highest nucleotide sequence identity of 71.9% with Tomato leaf curl Philippine betasatellite isolate Laguna1 (ToLCPB, AB307732). Phylogenetic analysis indicates that it is more related to isolate Laguna 1 and Laguna 2 of ToLCPB. According to the proposed species demarcation threshold of betasatellites (78% nucleotide identity), it is a novel betasatellite species, for which we propose the name Malvastrum leaf curl Guangdong betasatellite (MLCuGdB). |
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Keywords: | betasatellite
Begomovirus
Malvastrum leaf curl Guangdong virus
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