Histochemical quantification of dopa-oxidase (tyrosinase) activity in epidermal melanocytes |
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Authors: | Sasai Yoichiro Kumano Shuji Takahashi Masaaki Nakano Hiroyuki |
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Institution: | (1) Department of Dermatology, Kurume University School of Medicine, 830 Kurume, Japan;(2) Department of Dermatology, Tohoku University School of Medicine, Sendai, Japan |
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Abstract: | Summary The graphical method of Lineweaver-Burk was applied to the histochemical system to obtain quantitative information on the dopa-oxidase activity in human epidermal melanocytes. In vitro experiment showed that the incubation time necessary for yielding a certain amount of dopa-melanin is related to the amount of enzyme present. In the case of histochemical experiment, the time required for the first appearance of dopa-melanin deposition in melanocytes at various substrate concentrations was plotted against the reciprocal of the substrate concentration. This technique made it possible to compare the dopa-oxidase activity at various sites of the tissue. |
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