| 大肠杆菌工程菌ptsG基因敲除及其缺陷株混合糖同型乙醇发酵 |
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| 引用本文： | 严涛,赵锦芳,高文慧,王金华,王永泽,赵筱,周胜德. 大肠杆菌工程菌ptsG基因敲除及其缺陷株混合糖同型乙醇发酵[J]. 生物工程学报, 2013, 29(7): 937-945 |
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| 作者姓名： | 严涛 赵锦芳 高文慧 王金华 王永泽 赵筱 周胜德 |
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| 作者单位： | 工业发酵湖北省协同创新中心湖北工业大学生物工程学院,湖北武汉,430068 |
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| 基金项目： | 国家自然科学基金 (No. NSFC31070094)，湖北省教育厅科研项目 (No. Q20121405)，湖北省科技厅科研项目 (Nos. 2011CDA008，2011CDB076) 资助 |
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| 摘 要： | 为实现可同时利用木糖和葡萄糖进行生产发酵,以产乙醇的大肠杆菌工程菌SZ470为出发菌株(△pflB,△frdABCD,△ackA,△ldhA),采用同源重组技术,敲除葡萄糖转运基因ptsG,以构建不受葡萄糖抑制效应影响的菌株SZ470P.SZ470P在5％混合糖(2.5％木糖和2.5％葡萄糖)培养基中能同时利用葡萄糖和木糖进行发酵,葡萄糖消耗量是13 g/L,为对照菌株SZ470的一半；木糖消耗量是20 g/L,是SZ470的3.8倍；乙醇的最高产量为15.01 g/L,转化率为89.13％,比SZ470提高了14.32％.结果表明,工程菌SZ470P可同时利用葡萄糖和木糖发酵生产高产量的乙醇.
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| 关 键 词： | 大肠杆菌SZ470 ptsG基因 基因敲除 木糖发酵 葡萄糖效应 |
| 收稿时间： | 2012-12-23 |
Knockout of the ptsG gene in engineered Escherichia coli for homoethanol fermentation from sugar mixture |
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| Tao Yan,Jinfang Zhao,Wenhui Gao,Jinhua Wang,Yongze Wang,Xiao Zhao and Shengde Zhou. Knockout of the ptsG gene in engineered Escherichia coli for homoethanol fermentation from sugar mixture[J]. Chinese journal of biotechnology, 2013, 29(7): 937-945 |
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| Authors: | Tao Yan Jinfang Zhao Wenhui Gao Jinhua Wang Yongze Wang Xiao Zhao Shengde Zhou |
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| Affiliation: | College of Bioengineering, Hubei University of Technology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430068, Hubei, China;College of Bioengineering, Hubei University of Technology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430068, Hubei, China;College of Bioengineering, Hubei University of Technology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430068, Hubei, China;College of Bioengineering, Hubei University of Technology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430068, Hubei, China;College of Bioengineering, Hubei University of Technology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430068, Hubei, China;College of Bioengineering, Hubei University of Technology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430068, Hubei, China;College of Bioengineering, Hubei University of Technology, Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Wuhan 430068, Hubei, China |
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| Abstract: | To realize the simultaneous fermentation of xylose and glucose, ptsG (one of the glucose-PTS genes) was deleted from the engineered ethanologenic Escherichia coli SZ470 (?pflB, ?frdABCD, ?ackA, ?ldhA), resulting in loss of glucose effect in the mutant SZ470P (?ptsG). When tested in 50% mixture of glucose (2.5%) and xylose (2.5%), SZ470P simultaneously used glucose (13 g/L) and xylose (20 g/L) whereas the parent strain SZ470 sequentially used glucose (25?g/L) then xylose (5 g/L). Upon completion of the fermentation, both strains achieved similar product yield of 89%. SZ470P produced 15.01 g/L of ethanol, which was 14.32% higher than that produced by SZ470 (12.86 g/L). Deleting ptsG gene enabled the mutant strain SZ470P to simultaneously use both glucose and xylose and achieve better ethanol production |
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| Keywords: | Escherichia coli SZ470 ptsG gene gene knockout xylose fermentation glucose effect |
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