NAD + binding by yeast alcohol dehydro-genase in the presence of pyrazole and a new method for the determination of the enzyme active site concentration |
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| Authors: | Svetlana Trivic Jasmina Zeremski Vladimir Leskovac |
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| Institution: | (1) Faculty of Science, Novi Sad;(2) Faculty of Technology and Metallurgy Beograd, Bulevar Cara Lazara 1, YU-21000 Novi Sad, Yugoslavia;(3) Faculty of Technology Novi Sad, Bulevar Cara Lazara 1, YU-21000 Novi Sad, Yugoslavia |
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| Abstract: | The pyrazole method of Theorell and Yonetani ( Biochem. Z., 338: 573-553, 1963) has been adapted for the determination of the enzyme active site concentration in yeast alcohol dehydrogenase (EC 1.1.1.1). This method cannot be applied indiscriminately to other alcohol dehydrogenases without modification. |
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