Nicotiana tabacum actin-depolymerizing factor 2 is involved in actin-driven,auxin-dependent patterning |
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Authors: | Steffen Durst Peter NickJan Maisch |
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Institution: | Botanical Institute, Molecular Cell Biology, Karlsruhe Institute of Technology (KIT), Kaiserstraße 2, D-76131 Karlsruhe, Germany |
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Abstract: | Polar transport of auxin has been identified as a central element of pattern formation. To address the underlying cellular mechanisms, we use the tobacco cell line (Nicotiana tabacum L. cv. Bright Yellow 2; BY-2) as model. We showed previously that cell divisions within a cell file are synchronized by polar auxin flow, linked to the organization of actin filaments (AF) which, in turn, is modified via actin-binding proteins (ABPs). From a preparatory study for disturbed division synchrony in cell lines overexpressing different ABPs, we identified the actin depolymerizing factor 2 (ADF2). A cell line overexpressing GFP-NtADF2 was specifically affected in division synchrony. The cell division pattern could be rescued by addition of Phosphatidylinositol 4,5-bisphosphate (PIP2) or by phalloidin. These observations allow to draw first conclusions on the pathway linking auxin signalling via actin reorganization to synchronized cell division placing the regulation of cortical actin turnover by ADF2 into the focus. |
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Keywords: | ABP actin-binding protein ADF2 actin-depolymerizing factor 2 AF actin filaments BY-2 Nicotiana tabacum L cv Bright Yellow 2 suspension cell line MI mitotic index NPA 1-N-naphthylphthalamic acid NtADF2 ox BY-2 cell line overexpressing GFP-NtADF2 Lat B latrunculin B PIP2 Phosphatidylinositol 4 5-bisphosphate WT non-transformed BY-2 wild-type culture |
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