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| 高温加速老化过程中甜菜种子线粒体小分 | |
| 引用本文： | 宋松泉,lan M.Mφller.高温加速老化过程中甜菜种子线粒体小分[J].植物生理与分子生物学学报,2001,27(1). |
| 作者姓名： | 宋松泉 lan M.Mφller |
| 作者单位： | 1. 中山大学生命科学学院，中国广州510275;De-partment of Plant Physiology, Lund University, Box 117, S-22100 Lund, Sweden 2. Department of Seed Physiology, Novartis Seeds AB, Box 302, S-26123 L andskrona, Sweden 3. De-partment of Plant Physiology, Lund University, Box 117, S-22100 Lund, Sweden |
| 摘 要： | 甜菜(BetavulgarisLcv.Loke)种子的人工老化(30℃，100％RH)导致种子的干重、生活力和活力逐渐丧失，线粒体的呼吸速率、细胞色素c氧化酶和苹果酸脱氢酶的活性下降。线粒体蛋白质随着种子老化而显著变化，小分子量热休克蛋白(LMWHSP)22从老化的0d到30d增加，然后从30d到90d下降。可以认为甜菜种子人工老化过程中线粒体活性和LMWHSP22的含量变化与种子活力密切相关。 |
| 关 键 词： | 人工老化 甜菜 细胞色素c氧化酶 苹果酸脱氢酶 线粒体小分子量热休克蛋白22 呼吸速率 种子活力 |
Changes in Low-molecular Weight Heat Shock Protein 22 of Mitochon-dria During High-temperature Accelerated Ageing of Beta vulgaris L.Seeds |
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| Abstract: | High-temperature accelerated ageing (at 30℃ and 100% RH) of sugarbeet (Beta vulgaris L.cv. Loke ) seeds resulted in gradual loss of dry weight, viability and vigour of seeds, and lowering of mitochondrial respiratory rate and activities of cytochrome c oxidase and malate dehydrogenase in mitochondria. Protein composition in mitochondria showed marked changes with ageing of seeds; low-molecular weight heat shock protein (LMW HSP) 22 increased from 0 d to 30 d of ageing, and then decreased from 30 d to 90 d of ageing. It was found that changes in mitochondria activity and LMW HSP 22 content were closely correlated with seed vigour during accelerated ageing of sugar beet seeds. |
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