絮凝酵母颗粒悬浮床反应器中试及酒精连续发酵工艺的研究

在小型悬浮床反应器研究絮凝颗粒酵母酒精连续发酵工艺的基础上，建立了单釜反应器有效容积0．5m³、二级串联的中试装置。以淀粉糖化液为底物，考察了悬浮床反应器由小试到中试的放大效应，提出了该反应器工业放大时对危险截面表观气速应加以限制的原则，以保证絮凝酵母颗粒不被剪切破坏。进而在平均稀释率为0．10h^-1的条件下．中试装置稳定运转一个月，获得终点发酵液中酒精浓度80～85g／L，残糖低于5.0g／L，设备平均生产酒精8．4g／L·h的结果。

A study of Continous Ethanol Fermentation in Pilot plant Using Yeast Floes

Based on the bench scale experiment results using yeast floes from Schizosac-charamyces poinbe to produce ethanol from hydrolysate, a pilot plant composed of two suspended-bed bioreactors (SBB) in series with a total working volume of 1.0m3 was set up and had been operated over one months continuously. The SBBs were driven by air and limit oxygen was supplied, the floc concentration of 30~40g/L was maintained in suspended phase. It was discovered that the shearing effect of CO2 evolved on floes was not neglected during the scale-up of the SBB, and the superficial gas velocities on the dangerous cross-sections of the SBB should be controlled properly. At a dilution rate of 0.10h-1, the effluent contained ethanol 80-85g/L and residual sugar less than 5.0g/L, the average ethanol productivity was 8.4g/L ?h, and the conversion yield of sugar to ethanol was about 92% of the value theoretically obtainable.