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The effects of inoculum density and physical factors on the assessment of disease caused by Sclerotium rolfsii
Authors:J A Toribio  R Rodríguez-kábana
Institution:(1) Station de Pathologie Végétale, INRA, Centre Antilles-Guyane, B. P. 1232, 97184 Pointe-á-Pitre Cedex, Guadeloupe (West Indies);(2) Department of Plant Pathology, Auburn University, 36849 Auburn, AL, USA
Abstract:Greenhouse and laboratory experiments were conducted to determine the effects of various physical factors on the assessment of disease caused by Sclerotium rolfsii using field and artificially infested soils. Lentil(Lens esculenta Moench) seedlings growing in trays or pots with sand were inoculated by surrounding them with a layer of soil infested with the pathogen. The number of dead plants was maximal within a 10-day period following inoculation. Seedling mortality increased with the number of sclerotia in the soil to a maximum that depended on seedling spacing, depth of the soil layer, and soil type.
Keywords:Inoculum density            Lens esculenta            methodology            Sclerotium rolfsii            soil borne pathogens  southern blight
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