Size exclusion chromatography on soft and semi-rigid packing materials in the dynamic axial compression mode |
| |
| Authors: | Baru M B Danilov A V Vagenina I V |
| |
| Affiliation: | Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow Region 142290, Puschino, Russian Federation. synchro@fibkh.serpukhov.su |
| |
| Abstract: | The effect of dynamic axial compression within a range of up to 5 bar upon the structure of the bed packed with soft and semi-rigid packing materials (Sephadex G-25, Bio-Gel P2 and Toyopearl HW-40) and the associated chromatographic parameters were studied for size exclusion chromatography. Continuous packing compression is accomplished by use of a special column with controlled external pressure applied to the packing. Compression has been shown to favor an overall increase in the resolution with pressure optima observed in some cases. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
