首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Interaction of the HMG1 protein with nucleic acids
Authors:V A Shepelev
Institution:Institute of Molecular Genetics, USSR Academy of Sciences, 46 Kurchatov Sq., Moscow 123182. USSR
Abstract:Binding constants have been measured for the interaction of the protein HMG1 with native DNA, denatured DNA and a number of polynucleotides at near-physiological ionic strengths, using gel filtration and thermal denaturation. The interaction of HMG1 with DNA is shown to be noncooperative and reversible. Nucleic acids form the following series in order of increasing binding constants: poly(U) integral of poly(A) less than poly(dA) less than dsDNA integral of poly(dA) X poly(dT) integral of poly(dG) X poly(dC) much less than polyd(A-T]) integral of ssDNA.
Keywords:Nonhistone protein HMG1  DNA binding  Gel filtration  DNA melting  ssDNA  single-stranded DNA  dsDNA  double-stranded DNA  HMG1  high mobility group protein 1
本文献已被 ScienceDirect PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号