Interaction of the HMG1 protein with nucleic acids |
| |
Authors: | V A Shepelev |
| |
Institution: | Institute of Molecular Genetics, USSR Academy of Sciences, 46 Kurchatov Sq., Moscow 123182. USSR |
| |
Abstract: | Binding constants have been measured for the interaction of the protein HMG1 with native DNA, denatured DNA and a number of polynucleotides at near-physiological ionic strengths, using gel filtration and thermal denaturation. The interaction of HMG1 with DNA is shown to be noncooperative and reversible. Nucleic acids form the following series in order of increasing binding constants: poly(U) integral of poly(A) less than poly(dA) less than dsDNA integral of poly(dA) X poly(dT) integral of poly(dG) X poly(dC) much less than polyd(A-T]) integral of ssDNA. |
| |
Keywords: | Nonhistone protein HMG1 DNA binding Gel filtration DNA melting ssDNA single-stranded DNA dsDNA double-stranded DNA HMG1 high mobility group protein 1 |
本文献已被 ScienceDirect PubMed 等数据库收录! |