Formation of lipid peroxides in isolated rat liver microsomes by singlet molecular oxygen. |
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Authors: | M B Baird H R Massie M J Piekielniak |
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Affiliation: | Masonic Medical Research Laboratory, 2150 Bleecker Street, Utica, New York 13503 U.S.A. |
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Abstract: | Rat liver microsomes were incubated in neutral aqueous solution of potassium peroxychromate, a system which generates singlet molecular oxygen. Such incubation resulted both in a rapid decline in NADPH-cytochrome c reductase activity, and in an increase in formation of lipid peroxides. These reactions were not inhibited by either superoxide dismutase (SOD) or mannitol, nor were they entirely duplicated by incubating microsomes with hydrogen peroxide. However, a high concentration of 1,4-diazabicyclo-[2,2,2]octane (DABCO), a known scavenger of singlet oxygen, prevented both decline in reductase activity and formation of lipid peroxides. These results suggest that the observed effects are, in fact, attributable to singlet oxygen, and not to hydrogen peroxide, superoxide radical, or hydroxyl radical. |
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Keywords: | DABCO 1,4-diazobicyclo[2,2,2]octane SOD superoxide dismutase TBA thiobarbituric acid |
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