Morphogenetic effects of Brefeldin A on embryogenic cell cultures of Daucus carota L. |
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Authors: | Gloria Capitanio Barbara Baldan Francesco Filippini Mario Terzi Fiorella Lo Schiavo Paola Mariani |
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Institution: | (1) Department of Biology, University of Padua, Via Trieste 75, I-35121 Padova, Italy, IT |
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Abstract: | Brefeldin A, an inhibitor of protein secretion, caused typical alterations to the endomembrane system with limited effects
on viability when given to unorganized carrot cells growing in suspension. When given to the same cells during particular
stages of embryogenesis, it caused similar endomembrane lesions and an almost complete arrest of the embryogenic process.
Addition of conditioned medium containing extracellular secreted proteins to the embryos during treatment with Brefeldin A
allowed acquisition of polarity and the continuation of a quasi-normal embryogenic process.
Received: 4 December 1996 / Accepted: 4 March 1997 |
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Keywords: | : Brefeldin A Daucus Endomembrane syst-em Protein secretion Somatic embryogenesis |
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