Identification of oxindole-3-acetic acid,and metabolic conversion of indole-3-acetic acid to oxindole-3-acetic acid in Pinus sylvestris seeds |
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Authors: | Arild Ernstsen Göran Sandberg Karin Lundström |
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Institution: | (1) Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden |
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Abstract: | Oxindole-3-acetic acid (OxIAA) has been identified in germinating seeds of Scots pine (Pinus sylvestris) using gas chromatography-mass spectrometry. Seeds germinated for 5 d contained 2.7 ng OxIAA·g-1 (dry weight) whereas ungerminated seeds contained 0.2 ng·g-1. Isotopically labelled OxIAA was formed in seeds incubated with 1 -14C]-, 2 -14C]- or 2H5]indole-3-acetic acid.Abbreviations DDC
sodium diethyldithiocarbamate
- GC
gas chromatography
- HPLC
high-performance liquid chromatography
- IAA
indole-3-acetic acid
- MS
mass spectrometry
- OxIAA
oxindole-3-acetic acid
- PVP
polyvinylpyrrolidone
- TMS
trimethylsilyl |
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Keywords: | Auxin catabolism Indole-3-acetic acid catabolism Pinus Seed germination |
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