Ionic channels in the plasma membrane ofSchizosaccharomyces pombe: Evidence from patch-clamp measurements |
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| Authors: | Vladimír Vacata Milan Höfer H. Peter Larsson Harold Lecar |
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| Affiliation: | (1) Botanisches Institut der Universität Bonn, Kirschallee 1, 5300 Bonn 1, Germany;(2) Division of Biophysics and Cell Physiology, University of California, 94720 Berkeley, California |
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| Abstract: | Patch-clamp studies of the yeastSchizosaccharomyces pombe reveal that the plasma membrane contains a voltage-gated channel mildly selective for potassium over sodium, lithium, and chloride. The channel exhibits several conductances with a maximum of 153 pS. The channel gates in the region of physiologically relevant voltages, being closed at hyperpolarizing and open at depolarizing voltages. It is not inhibited by tetraethylammonium, quinine, or quinidine applied from the cytoplasmic side of the membrane; similarly, ATP and stretch have no effect. The frequency of its occurrence in patches implies that about 35 channels of this kind are present in the plasma membrane of a single cell. |
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| Keywords: | Plasma membrane channels channel gating multilevel conductance ionic selectivity density of channels Schizosaccharomyces pombe |
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